Figure 2. Cosmc transfection downregulated Tn+ cell proliferation and migration. (A–F) Proliferation and migration in LS174T-Tn− and LS174T-Tn+ cells before and after transfection with WtCosmc or Mock were detected by RTCA. (A) Proliferation curve, (B) Migration curve, (C) Cell index of proliferation at typical timepoints (30 h and 60 h), (D) Slopes for proliferation at 0–60 h, (E) Cell index of migration at typical timepoints (12 h and 24 h), and (F) slopes for migration at 0–24 h. (G) The proliferation ability of Jurkat T cells transfected with Cosmc or Mock was detected by CCK-8 assay at different timepoints (15 h, 30 h, 45 h, and 60 h). (H) The migration of Jurkat T cells transfected with Cosmc or Mock was detected using a transwell assay for 24 h. Data shown are the mean ± SD of three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001).