Research Paper Volume 13, Issue 19 pp 23393—23406

Cosmc transfection decreases malignant behavior of Tn+ cells and enhances sensitivity to apoptosis when induced by Apo2L/TRAIL via alteration of O-glycan structure

Apoptosis of Tn+ cells analysis with FCM before and after treatment with WtCosmc and Apo2L/TRAIL. (A–B) Apoptosis rates in LS174T-Tn+ and LS174T-Tn− cells. (C–D) Apoptosis rates in LS174T-Tn+-Mock and LS174T-Tn+-Cosmc cells. (E–F) Apoptosis rates in Jurkat T, Jurkat T-Mock, and Jurkat T-Cosmc cells. (G) Apoptosis of LS174T-Tn− and LS174T-Tn+ cells after exposure to Apo2L/TRAIL at different concentrations (10 ng/mL, 50 ng/mL, and 100 ng/mL) before and after transfection with WtCosmc or Mock. (H) Apoptosis of Jurkat T cells after exposure to Apo2L/TRAIL at different concentrations (10 ng/mL, 20 ng/mL, and 30 ng/mL) before and after transfection with WtCosmc or Mock. (I) The apoptosis of LS174T-Tn− and LS174T-Tn+ cells were exposed to Apo2L/TRAIL at 100 ng/mL and different timepoints (6 h, 12 h, and 18 h) before and after transfection with WtCosmc or Mock. (J) Apoptosis of Jurkat T cells after exposure to Apo2L/TRAIL at 20 ng/mL and different timepoints (6 h, 12 h, and 18 h) before and after transfection with WtCosmc or Mock. Data shown are the mean {plus minus} SD of three independent experiments (***P

Figure 3. Apoptosis of Tn+ cells analysis with FCM before and after treatment with WtCosmc and Apo2L/TRAIL. (AB) Apoptosis rates in LS174T-Tn+ and LS174T-Tn cells. (CD) Apoptosis rates in LS174T-Tn+-Mock and LS174T-Tn+-Cosmc cells. (EF) Apoptosis rates in Jurkat T, Jurkat T-Mock, and Jurkat T-Cosmc cells. (G) Apoptosis of LS174T-Tn and LS174T-Tn+ cells after exposure to Apo2L/TRAIL at different concentrations (10 ng/mL, 50 ng/mL, and 100 ng/mL) before and after transfection with WtCosmc or Mock. (H) Apoptosis of Jurkat T cells after exposure to Apo2L/TRAIL at different concentrations (10 ng/mL, 20 ng/mL, and 30 ng/mL) before and after transfection with WtCosmc or Mock. (I) The apoptosis of LS174T-Tn and LS174T-Tn+ cells were exposed to Apo2L/TRAIL at 100 ng/mL and different timepoints (6 h, 12 h, and 18 h) before and after transfection with WtCosmc or Mock. (J) Apoptosis of Jurkat T cells after exposure to Apo2L/TRAIL at 20 ng/mL and different timepoints (6 h, 12 h, and 18 h) before and after transfection with WtCosmc or Mock. Data shown are the mean {plus minus} SD of three independent experiments (***P < 0.001).