Research Paper Volume 13, Issue 19 pp 23393—23406

Cosmc transfection decreases malignant behavior of Tn+ cells and enhances sensitivity to apoptosis when induced by Apo2L/TRAIL via alteration of O-glycan structure

Cosmc transfection increased the activity of T-synthase and changed core 1− and core 2-derived O-glycans in Tn+ cells. (A) The activity of T-synthase was detected using a fluorescence method in LS174T-Tn−, LS174T-Tn+, and Jurkat T cells before and after transfection with WtCosmc or Mock. (B) Core 1- and core 2-derived O-glycans in Jurkat T cells before and after transfection with WtCosmc or Mock were analyzed using CORA and MALDI-TOF-MS. (C) Core 1− and core 2-derived O-glycans in LS174T-Tn− and LS174T-Tn+ cells before and after transfection with WtCosmc or Mock (**P ***P

Figure 4. Cosmc transfection increased the activity of T-synthase and changed core 1 and core 2-derived O-glycans in Tn+ cells. (A) The activity of T-synthase was detected using a fluorescence method in LS174T-Tn, LS174T-Tn+, and Jurkat T cells before and after transfection with WtCosmc or Mock. (B) Core 1- and core 2-derived O-glycans in Jurkat T cells before and after transfection with WtCosmc or Mock were analyzed using CORA and MALDI-TOF-MS. (C) Core 1 and core 2-derived O-glycans in LS174T-Tn and LS174T-Tn+ cells before and after transfection with WtCosmc or Mock (**P < 0.01, ***P < 0.001).