Research Paper Volume 13, Issue 19 pp 23393—23406

Cosmc transfection decreases malignant behavior of Tn+ cells and enhances sensitivity to apoptosis when induced by Apo2L/TRAIL via alteration of O-glycan structure

Cosmc transfection did not influence the activity of C3GnT and core 3-derived O-glycans in Tn+ cells. (A) The procedure of fluorescence detection of C3GnT activity. (B) The activity of C3GnT was detected using a fluorescence method in LS174T-Tn−, LS174T-Tn+, and Jurkat T cells before and after transfection with WtCosmc or Mock. (C) Core 3-derived O-glycans in Jurkat T cells before and after transfection with WtCosmc or Mock were analyzed using CORA and MALDI-TOF-MS. (D) Core 3-derived O-glycans in LS174T-Tn− and LS174T-Tn+ cells before and after transfection with WtCosmc or Mock (***P

Figure 5. Cosmc transfection did not influence the activity of C3GnT and core 3-derived O-glycans in Tn+ cells. (A) The procedure of fluorescence detection of C3GnT activity. (B) The activity of C3GnT was detected using a fluorescence method in LS174T-Tn, LS174T-Tn+, and Jurkat T cells before and after transfection with WtCosmc or Mock. (C) Core 3-derived O-glycans in Jurkat T cells before and after transfection with WtCosmc or Mock were analyzed using CORA and MALDI-TOF-MS. (D) Core 3-derived O-glycans in LS174T-Tn and LS174T-Tn+ cells before and after transfection with WtCosmc or Mock (***P < 0.001).