Research Paper Advance Articles

Nmnat2 attenuates amyloidogenesis and up-regulates ADAM10 in AMPK activity-dependent manner

Nmnat2 attenuates amyloidogenesis and up-regulates ADAM10 in AMPK activity-dependent manner in N2a/APPswe cells. (A–C) N2a/APPswe cells were treated with 2 mM AICAR for twenty four hours, and then ADAM10 was detected by western blot (A) and quantitative analysis (B). The medium of N2a/APPswe cells treated with 2 mM AICAR for twenty four hours was measured for Aβ1-40 and Aβ1-42 by using ELISA (C). N2a/APPswe cells were transfected with Flag-Nmnat2 (Nmnat2) or empty vector for twenty four hours, and then treated with 20 μM Compound C (CC) for another twenty four hours. DMSO was used as the vehicle control of CC treatment. And then ADAM10 was measured by western blot (D) and quantitative analysis (E). The medium of N2a/APPswe cells transfected with Nmnat2 and CC treatment was detected for Aβ1-40 and Aβ1-42 by ELISA (F). The data were representative from three independent experiments at least and expressed as means ± S.D.. *P P P P

Figure 7. Nmnat2 attenuates amyloidogenesis and up-regulates ADAM10 in AMPK activity-dependent manner in N2a/APPswe cells. (AC) N2a/APPswe cells were treated with 2 mM AICAR for twenty four hours, and then ADAM10 was detected by western blot (A) and quantitative analysis (B). The medium of N2a/APPswe cells treated with 2 mM AICAR for twenty four hours was measured for Aβ1-40 and Aβ1-42 by using ELISA (C). N2a/APPswe cells were transfected with Flag-Nmnat2 (Nmnat2) or empty vector for twenty four hours, and then treated with 20 μM Compound C (CC) for another twenty four hours. DMSO was used as the vehicle control of CC treatment. And then ADAM10 was measured by western blot (D) and quantitative analysis (E). The medium of N2a/APPswe cells transfected with Nmnat2 and CC treatment was detected for Aβ1-40 and Aβ1-42 by ELISA (F). The data were representative from three independent experiments at least and expressed as means ± S.D.. *P < 0.05, **P < 0.01 vs control; #P < 0.05, ##P < 0.01 vs Nmnat2.