Research Paper Volume 13, Issue 20 pp 23726—23738

Exosome long non-coding RNA SOX2-OT contributes to ovarian cancer malignant progression by miR-181b-5p/SCD1 signaling

SOX2-OT serves as a miR-181b-5p sponge in ovarian cancer cells. (A) The interaction of SOX2-OT and miR-181b-5p was analyzed by bioinformatic analysis based on ENCORI (<a href="http://starbase.sysu.edu.cn/index.php" target="

Figure 4. SOX2-OT serves as a miR-181b-5p sponge in ovarian cancer cells. (A) The interaction of SOX2-OT and miR-181b-5p was analyzed by bioinformatic analysis based on ENCORI (http://starbase.sysu.edu.cn/index.php). (B) The expression levels of miR-181b-5p were assessed by qPCR in the TOV-21G and SKOV-3 cells treated with control mimic or miR-181b-5p mimics. (C, D) Luciferase activities of SOX2-OT (SOX2-OT WT) and SOX2-OT with the miR-181b-5p-binding site mutant (SOX2-OT MUT) were determined by luciferase reporter gene assays in the TOV-21G and SKOV-3 cells treated with control mimic or miR-181b-5p mimic. (E) The TOV-21G and SKOV-3 cells were treated with control shRNA or SOX2-OT shRNA. The expression of miR-181b-5p was measured by qPCR assays in the cells. Data are presented as mean ± SD. Statistic significant differences were indicated: ** P < 0.01.