Figure 5. MiR-20a-5p can target SOX4 in T-ALL cells. (A) The predicted interaction site between circPRKCI and miR-20a-5p. (B–F) MOLT-4 and JURKAT cells were treated with miR-20a-5p mimic. (B, C) The treatment efficacy of miR-20a-5p mimic in MOLT-4 and JURKAT cells was determined by qRT-PCR assay. (D, E) The luciferase activity of SOX4 was determined by dual luciferase reporter assay. (F) The expression of SOX4 was determined by Western blot analysis. (G) MOLT-4 and JURKAT cells were treated with circPRKCI shRNA or co-treated with miR-20a-5p inhibitor. The expression of SOX4 was analyzed by Western blot analysis. **p < 0.01.