Research Paper Volume 13, Issue 20 pp 23780—23795

Matairesinol exerts anti-inflammatory and antioxidant effects in sepsis-mediated brain injury by repressing the MAPK and NF-κB pathways through up-regulating AMPK

Mat attenuated the LPS-mediated neuronal damage. Different concentrations (0 to 80 μg/mL) of Mat were taken to treat NSC-34 and HT22 neurons for 48 hours. (A) The toxicity of Mat on neurons was gauged by MTS analysis. On the basis of 1 μg/mL LPS treatment for 24 hours, different concentrations (5 to 20 μg/mL) of Mat were taken to treat NSC-34 and HT22 cells for 48 hours. (B) Cell proliferation was assessed by MTS analysis at the 24th, 48th and 72nd hours after Mat treatment. (C–D) Apoptosis was examined by FCM. (E) WB verified the expression of Caspase3 and Bcl2. *P ***P +P ++P ++P

Figure 1. Mat attenuated the LPS-mediated neuronal damage. Different concentrations (0 to 80 μg/mL) of Mat were taken to treat NSC-34 and HT22 neurons for 48 hours. (A) The toxicity of Mat on neurons was gauged by MTS analysis. On the basis of 1 μg/mL LPS treatment for 24 hours, different concentrations (5 to 20 μg/mL) of Mat were taken to treat NSC-34 and HT22 cells for 48 hours. (B) Cell proliferation was assessed by MTS analysis at the 24th, 48th and 72nd hours after Mat treatment. (CD) Apoptosis was examined by FCM. (E) WB verified the expression of Caspase3 and Bcl2. *P < 0.05, ***P < 0.001 (vs. control group). +P < 0.05, ++P < 0.01, ++P < 0.001 (vs. LPS group).