Priority Research Paper Volume 13, Issue 21 pp 23876—23894

Alteration of mitochondrial homeostasis is an early event in a C. elegans model of human tauopathy

Impaired mitochondrial homeostasis and motility defects in tauwt-expressing 3-day adult animals. (A) Age-dependent accumulation of wild type tau protein in neuronal cells (heigh molecular weight; HMW). (B) Representative fluorescent images of transgenic nematodes expressing pan-neuronally mitochondria-targeted mKate2::HA. Scale bar, 20 μm. Tauwt-expressing 3-day-adult nematodes display (C) increased mitochondrial population and (D) smaller organelle compared to their wild type counterparts (n = 30; ***P t-test). (E, F) Tauwt expressing nematodes display decreased mitochondrial membrane potential (n = 40; ***P t-test). Scale Bar, 100 μm. (G) Representative images of 3-day adult wild type and tauwt expressing nematodes tracks. (H) Body bends of 3-day adult wild type and tauwt- animals per 30 seconds in M9 buffer (n = 20; ***P t-test).

Figure 1. Impaired mitochondrial homeostasis and motility defects in tauwt-expressing 3-day adult animals. (A) Age-dependent accumulation of wild type tau protein in neuronal cells (heigh molecular weight; HMW). (B) Representative fluorescent images of transgenic nematodes expressing pan-neuronally mitochondria-targeted mKate2::HA. Scale bar, 20 μm. Tauwt-expressing 3-day-adult nematodes display (C) increased mitochondrial population and (D) smaller organelle compared to their wild type counterparts (n = 30; ***P < 0.0001; unpaired t-test). (E, F) Tauwt expressing nematodes display decreased mitochondrial membrane potential (n = 40; ***P < 0.0001; unpaired t-test). Scale Bar, 100 μm. (G) Representative images of 3-day adult wild type and tauwt expressing nematodes tracks. (H) Body bends of 3-day adult wild type and tauwt- animals per 30 seconds in M9 buffer (n = 20; ***P < 0.0001; unpaired t-test).