Priority Research Paper Volume 13, Issue 21 pp 23876—23894

Alteration of mitochondrial homeostasis is an early event in a C. elegans model of human tauopathy

Altered mitochondrial morphology and activity in tauwt-expressing larvae. Wild type and tauwt L1 (A) and L4 (B) larvae body bends per 30 seconds in M9 buffer. (C) Mitochondrial population in the ventral nerve cord of L1 nematodes. (D) Representative fluorescent images of transgenic nematodes expressing pan-neuronally mitochondria-targeted mKate2::HA. Scale bar, 20 μm. Tauwt-expressing L1 larvae display (E) smaller, (F, G) more circular and (H) Fewer active mitochondria compared to wild type animals. Scale bar, 100 μm. (n = 50; NS P > 0.05, **P ***P t-test).

Figure 2. Altered mitochondrial morphology and activity in tauwt-expressing larvae. Wild type and tauwt L1 (A) and L4 (B) larvae body bends per 30 seconds in M9 buffer. (C) Mitochondrial population in the ventral nerve cord of L1 nematodes. (D) Representative fluorescent images of transgenic nematodes expressing pan-neuronally mitochondria-targeted mKate2::HA. Scale bar, 20 μm. Tauwt-expressing L1 larvae display (E) smaller, (F, G) more circular and (H) Fewer active mitochondria compared to wild type animals. Scale bar, 100 μm. (n = 50; NS P > 0.05, **P < 0.001, ***P < 0.0001; unpaired t-test).