TIMP1 is upregulated in human gastric cancer. (A, B) Immunohistochemistry of TIMP1 expression in 42 gastric carcinoma tissues and 42 adjacent normal gastric tissues. Representative immunohistochemistry images (A) and semi-quantitative evaluation (B) of TIMP1protein expression. (C, D) Analysis of TIMP1 expression in 42 gastric carcinoma tissues and 42 adjacent normal gastric tissues. Representative western blotting images of TIMP1 protein levels in three normal gastric tissues and three gastric carcinoma tissues (C). TIMP1 and GAPDH protein levels were determined via densitometry using ImageJ and are represented as IOD (D). (E) Kaplan-Meier survival analyses of GC patients with high or low TIMP1 expression based on KM plotter database and GSE15459 dataset Data represent the means ± SEM. **P < 0.01. ns, not significant.