Research Paper Volume 13, Issue 22 pp 24829—24849

Bone morphogenetic protein 9 enhances osteogenic and angiogenic responses of human amniotic mesenchymal stem cells cocultured with umbilical vein endothelial cells through the PI3K/AKT/m-TOR signaling pathway


Figure 1. Characterization of isolated hAMSCs and HUVECs. hAMSCs at P0 to P3 showing a fibroblast-like morphology and spindle shaped configuration (×40, bar: 200 μm) (A); CCK-8 assay showed the doubling time for hAMSCs and HUVECs was 28 h and 33.5 h, respectively (*P < 0.05) (B); Flow cytometry results showed phenotypical identities of hAMSCs at P3 (C); Phenotypic properties of HUVECs and immunofluorescence staining showed HUVECs at P3 highly expressed the surface markers of endothelial cells (×100, bar: 50 μm) (D); Multi-lineage differentiation potential of hAMSCs in vitro. Alizarin Red S staining of hAMSCs for 21 d. Alcian Blue staining of hAMSCs for 21 d. Oil Red O staining of hAMSCs for 21 d (×100, bar: 50 μm) (E); hAMSCs at P3 scarcely expressed CK-19 and highly expressed vimentin. CK-19 and vimentin were stained green by FTIC, and cell nuclei were stained blue by 4’, 6-diamidino-2-phenylindole (DAPI) (×100, bar: 50μm) (F). FITC: Fluorescein isothiocyanate; PE: Phycoerythrin.