Research Paper Volume 13, Issue 22 pp 24829—24849

Bone morphogenetic protein 9 enhances osteogenic and angiogenic responses of human amniotic mesenchymal stem cells cocultured with umbilical vein endothelial cells through the PI3K/AKT/m-TOR signaling pathway


Figure 3. BMP9 upregulates the expression of osteogenic and angiogenic related factors of cocultured hAMSCs and HUVECs in vitro. RT-qPCR assay was performed to detect the mRNA expression level of BMP9 induced osteogenic relative factors COL-I, Runx2, OPN, and BSP in each group at 5 d (A); The mRNA expression level of BMP9 induced angiogenic relative factors including ANT1, VEGF, CD31, and vWF in each group at 5 d (B); Western blot was used to determine the protein expression of OPN (C), OCN (D), and Runx2 (E) of hAMSCs cocultured HUVECs at 5 d in vitro; Quantification analysis of Western blot showed that BMP9 significantly increased the protein expression of osteogenic relative factors OPN, OCN, and Runx2 in hAMSCs cocultured with HUVECs at days 5 (F) (*P < 0.05).