Research Paper Volume 13, Issue 22 pp 24829—24849

Bone morphogenetic protein 9 enhances osteogenic and angiogenic responses of human amniotic mesenchymal stem cells cocultured with umbilical vein endothelial cells through the PI3K/AKT/m-TOR signaling pathway


Figure 5. Immunofluorescence staining and quantification analysis of osteogenic and angiogenic differentiation of cocultured hAMSCs and HUVECs in vivo. Immunofluorescence and quantified analysis of ectopic bone masses to investigate the synthesis of osteogenic relative factors OPN (green) and VEGF (green) (×400, bar: 20 μm) (A); The protein expression of OPN and VEGF were analyzed by immunofluorescence and quantified analysis (B) (*P < 0.05).