Research Paper Volume 13, Issue 23 pp 25180—25194
Licochalcone A activation of glycolysis pathway has an anti-aging effect on human adipose stem cells
Figure 1. Effects of LA on hADSC viability. (A) SA-β-gal staining of hADSCs (Scale bar: 100 μm). The relative intensity of ROS was detected by a microplate reader. (B) Effects of LA on ROS. (C) Relative telomere lengths of hADSCs. (D) Relative protein expression of senescence gene P16 compared with the control. (E) Effects of LA on cell cycle. (F, G) Relative mRNA expression of senescent genes P21 and P53 compared with control. Data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 compared with untreated cells.