Research Paper Volume 13, Issue 23 pp 25180—25194
Licochalcone A activation of glycolysis pathway has an anti-aging effect on human adipose stem cells
Figure 2. Effects of LA on hADSC differentiation ability. (A) qRT-PCR of mRNA levels of the osteogenic differentiation markers ALP, OCN, and RUNX2. (B) Alizarin Red staining of hADSCs. (C) Western blot in hADSCs treated as indicated. (D) qRT-PCR of mRNA levels of the adipogenic differentiation markers PPARr and LPL. (E) Oil Red O staining of hADSCs. (F) Western blot in hADSCs treated as indicated. Data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 compared with untreated cells.