Research Paper Volume 13, Issue 23 pp 25180—25194

Licochalcone A activation of glycolysis pathway has an anti-aging effect on human adipose stem cells


Figure 3. Mechanism of LA in regulation of hADSC proliferation determined by RNA-seq. (A) Classification map of KEGG pathway analysis. (B) Bubble diagram of glycolysis/gluconeogenesis signaling pathways regulated by LA. (C) Heatmap of differentially expressed genes related to glycolysis/gluconeogenesis signaling pathways. (D) The differentially expressed genes. (E) Enrichment plots of gene expression signatures for the glycolysis/gluconeogenesis signaling pathway. (F) qRT-PCR of the relative mRNA expression of PKM, PFKP, and GAPDH in LA-treated hADSCs. Data are presented as the mean ± SD of three independent experiments. Statistical significance was determined by the unpaired t-test. *p < 0.05, **p < 0.01 compared with the Ctrl.