Figure 5. Immunofluorescent staining images of lymphatic vessels of the patient's skin. Immunofluorescent staining images of lymphatic vessels in the dermis of two patients with WS and one with PAD are shown. A stained image with WRN antibody, Hoechst 33342, superimposed image, αSMA antibody, and podoplanin antibody are shown on the left. Samples collected from PAD1, WS1, and WS4 are shown at the top. In PAD1, the WRN protein is localized in the nucleus (arrow), whereas in WS1 and WS4, it is spread throughout the cytoplasm.