Research Paper Volume 14, Issue 3 pp 1265—1279
Hesperidin suppresses ERS-induced inflammation in the pathogenesis of non-alcoholic fatty liver disease
Figure 5. HSP attenuates ERS in human THP-1 cells. As described in the method, FFA-stimulated human THP-1 cells were employed to establish the NAFLD model. Thapsigargin is a well-described ERS inducer successfully used in various cell lines. (A) Western blotting analysis was performed to examine the expression of GRP94, ATF6, ATF4, p-PERK, and p-IRE1α in human THP-1 cells. (B) Western blotting analysis was performed to examine the expression of IL-1β, IL-6, and TNF-α.