Figure 3. PAX5 transcriptionally activates and enhances PSMA3-AS1 expression. (A) JASPAR database detected the binding sites of PAX5 and PSMA3-AS1. (B) Knockdown efficiency and amplification efficiency of PAX5 in CCA cells both at mRNA and (C) protein level. (D) PAX5 regulated PSMA3-AS1 expression. (E) The expression of PAX5 in CCA tissues both at mRNA and (F) protein level. (G) PAX5 expression was positively correlated with PSMA3-AS1 expression. (H) The expression of PAX5 in CCA cells both at mRNA and (I) protein level. (J) ChIP assay confirmed that the binding site 1 of PSMA3-AS1 promoter was responsive to PAX5-induced transcription, but no obvious changes in binding site 2. (K) Luciferase reporter assay confirmed that the luciferase activity of the wild type of binding site 1 was increased by elevating PAX5, but no significant change in mutant type group. **P < 0.01, ***P < 0.001.