Research Paper Volume 14, Issue 1 pp 509—525

Figure 8. PSMA3-AS1 up-regulates LAMC1 by sponging miR-376a-3p to accelerate CCA development. (A, B) Knockdown of PSMA3-AS1 inhibited LAMC1 expression in CCA cells, but co-transfection of miR-376a-3p inhibitor rescued this process. On the contrary, overexpression of PSMA3-AS1 promoted LAMC1 expression, whereas co-transfection of miR-376a-3p mimics could save the promoting effect caused by PSMA3-AS1 overexpression. (C–F) Knocking down miR-376a-3p could reverse the inhibition of proliferation, invasion and EMT caused by PSMA3-AS1 knockdown. (G–J) Downregulated LAMC1 could rescue the promotion of proliferation, invasion and EMT generated by PSMA3-AS1 overexpression. (K–N) Knocking down miR-376a-3p promoted the proliferation, invasion and EMT process of CCA cells, however, these promotive effects were attenuated by LAMC1 knockdown. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.