Research Paper Volume 14, Issue 3 pp 1321—1335
CTRP3 alleviates cardiac ischemia/reperfusion injury via LAMP1/JIP2/JNK signaling pathway
Figure 4. Effect of LAMP1 overexpression on OGD/R injury in H9C2 cells. H9C2 cells were transfected with pcDNA-LAMP1 or negative control (vector) for 24 h and then exposed to OGD/R injury. (A) Relative protein expression of LAMP1was detected by Western blotting. (B, C) Effect of LAMP1 overexpression on cell proliferation was assessed by using CCK-8 and EdU assays. (D) Effect of LAMP1 overexpression on cell apoptosis was detected by flow cytometry. (E–G) Effects of CTRP3 overexpression on ROS, MDA and cTn-I production were determined.