Research Paper Volume 14, Issue 10 pp 4305—4325
MRTF-A-mediated protection against amyloid-β-induced neuronal injury correlates with restoring autophagy via miR-1273g-3p/mTOR axis in Alzheimer models
Figure 4. MRTF-A attenuated Aβ-induced neurotoxicity by promoting autophagy in SH-SY5Y cells. (A, B) The autophagy flux analysis using Ad-mCherry-GFP-LC3B-tagged protein. (C, D) Western blot analysis protein for Beclin1 and p62 expression. (E, F) TUNEL staining for cell apoptosis in Aβ-treated SH-SY5Y cells followed MRTF-A treatment co-incubation with or without 3-MA. Data represent means ± SEM of 3 independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 (one-way ANOVA, Tukey’s Multiple Comparison Test).