Figure 2. circSHPRH knockdown promotes BCa cell proliferation, migration and invasion. (A) si-circSHPRH was specifically designed to target the back-splice site of circSHPRH. (B, C) The relative expression of circSHPRH and SHPRH in T24 and UM-UC-3 cells transfected with si-NC or si-circSHPRH. (D, E) The relative expression of SHPRH and circSHPRH in T24 and UM-UC-3 cells transfected with si-NC or si-SHPRH. (F, G) circSHPRH knockdown enhanced cell viability in T24 and UM-UC-3 cells, as shown by the MTS assay. (H, I) circSHPRH knockdown promoted the migration ability of T24 and UM-UC-3 cells, as measured by wound healing assay. (J–L) circSHPRH knockdown promoted the invasion ability of T24 and UM-UC-3 cells, as detected by Transwell invasion assay. Magnification, 200×, *P<0.05, **P<0.01.