Figure 3. circSHPRH directly binds to miR-942. (A, B) circSHPRH was predominantly located in the cytoplasm of T24 cells, as detected by nucleus-plasma separation assay (A) and FISH (B). (C) Eighteen miRNAs possess potential circSHPRH binding sites, as predicted by CircInteractome. (D) miR-942 was pulled down and enriched with a circSHPRH specific probe in both T24 and UM-UC-3 cells, as detected by qRT–PCR. (E) The relative expression of miR-942 in T24 and UM-UC-3 cells transfected with the vector or circSHPRH plasmid. (F) The potential circSHPRH binding site and mutant sequence in miR-942. (G) circSHPRH was captured by miR-942 using a biotin-labeled miRNA capture assay. (H) The potential miR-942 binding site and mutant sequence in circSHPRH. (I) Luciferase activity was detected in T24 and UM-UC-3 cells transfected with wild-type (wt) circSHPRH (or mutant) and miR-942 mimics or mimics-NC. *P<0.05, **P<0.01.