Figure 2. Exploration of the relationship between AK4P1 and cognate genes in PDAC. (A) Two cognate genes (AK3 and AK4) of pseudogene AK4P1. (B) The distribution of AK3/4 gene in different cell types of human normal pancreas was confirmed by scRNA-seq. (C) The expression level of the AK3/4 gene in different cell types of normal pancreas was determined by scRNA-seq. (D) The mRNA levels of AK3/4 expression in PDAC and normal pancreas tissues were determined by RNA-seq data from GEPIA. (E) The protein levels of AK3/4 expression in PDAC and normal pancreas tissues were determined by immunohistochemical staining data from HPA. (F) The expression correlation between AK4P1 and its cognate genes in PDAC was assessed using GEPIA. (G) Expression of AK3/4 genes among major stages in PDAC. (H) Prognostic role (OS) of AK3/4 genes in PDAC. (I) Prognostic role (DFS) of 5 potential DEPs in PDAC. Three horizontal lines in the box plot represent minimum, median and maximum, respectively; scale bar, 100 mm; *P < 0.05.