Figure 4. SIRT1 is involved in the function of human GCs. (A) SIRT1 was downregulated in the 3-NPA model group in immunohistochemistry (Triplicates slides in each group). (B) SIRT1 was downregulated in the 3-NPA model group in western blot (Quadruplicates samples in each group). (C, D) SIRT1 was knocked down using a lentivirus. (E) Estrogen synthesis decreased after SIRT1 knockdown. (F) Genes related to estrogen synthesis were down-regulated after SIRT1 knockdown. (G) Functional receptors were down-regulated in GCs after SIRT1 knockdown (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001).