Priority Research Paper Volume 14, Issue 6 pp 2442—2461

Regulation of mTOR complexes in long-lived growth hormone receptor knockout and Snell dwarf mice

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Figure 3. Decreased DEPTOR and PRAS40 in immunoprecipitated mTOR complexes. (A) Representative immunoblots of the mTORC1 components from samples immunoprecipitated with anti-RAPTOR antibody. (B) Representative immunoblots of the mTORC2 components immunoprecipitated with anti-RICTOR antibody. (C) Representative immunoblots of the input samples. (D) Quantification of the protein levels in RAPTOR-IP samples, as in (A). (E) Quantification of the protein levels in RICTOR-IP samples, as in (B), for N = 6 male mice, with mean and SEM. *t-test p < 0.05.