Research Paper Volume 14, Issue 7 pp 3313—3324
SNP rs322931 (C>T) in miR-181b and rs7158663 (G>A) in MEG3 aggravate the inflammatory response of anal abscess in patients with Crohn’s disease
Figure 7. The successful transfection of p-MEG3, which was validated by the evidently enhanced expression of MEG3 (A), inhibited the expression of miR-181b (B) and increased the expression of TNF-α mRNA (C) and protein (D). Moreover, the successful transfection of p-anti-miR-181b, which was validated by the evident inhibition of miR-181b expression (B), promoted the expressions of MEG3 (A), TNF-α mRNA (C) and protein (D) (* P value < 0.05 vs. Empty vector group; 3 biological repeats).