Research Paper Volume 14, Issue 10 pp 4486—4499

Figure 6. KCNQ1OT1 inhibits osteoclast differentiation by upregulating NFAT5 expression via sponging miR-128-3p. (A–F) RAW 264.7 cells were transfected with pcDNA-KCNQ1OT1, miR-128-3p mimic or pcDNA-KCNQ1OT1 + miR-128-3p mimic respectively, and then treated with RANKL for 5 days. qRT-PCR and Western blotting showed the mRNA and protein expression levels of NFAT5, c-Fos, NFATc1, and Ctsk. (G–L) Similar experiments were also performed in RAW 264.7 cells transfected with si-KCNQ1OT1, miR-128-3p inhibitor or si-KCNQ1OT1 + miR-128-3p inhibitor. (M) Multinucleated osteoclasts were stained by TRAP and then counted (Scale bar: 200 μm). ^*P < 0.05, ^**P < 0.01; ^***P < 0.001, ns: not significant.