Research Paper Volume 14, Issue 18 pp 7364—7377

Figure 7. Effect of M1-Exo-GEM on tumor apoptosis in tumor-bearing mice. (A, B) In the protein assay, Bcl-2 was overexpressed in the Control group, while Caspase-3 and Bax were under expressed. GEM and M1-Exo enhanced the expressions of Caspase-3 and Bax and inhibited the Bcl-2expression, suggesting their pro-apoptosis activities. M1-Exo-GEM could further promote the apoptosis signal activation. *P<0.05 vs. Control group. (B–E) In the detection of tissue inflammatory cytokines, the TNF-α, IL-6 and IL-1β were lowly expressed in the Control group. M1-Exo and GEM enhanced the expressions of these inflammatory cytokines, whose levels were significantly higher than the Control group. M1-Exo-GEM could further up-regulate the inflammatory cytokine levels. *P<0.05 vs. Control group. (F) According to the H&E staining results for pathological examination of mouse heart, liver, lung and kidney, M1-Exo-GEM had no toxic effect on other organs. No obvious pathological changes were observed in the organs or tissues, which were in a normal physiological state.