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Research Paper Volume 14, Issue 18 pp 7378—7389

Fasudil inhibits hepatic artery spasm by repressing the YAP/ERK/ ETA/ETB signaling pathway via inhibiting ROCK activation

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Figure 5. Fasudil suppressed the YAP/ERK/ETA/ETB signaling pathway in HA spasm by inhibiting ROCK activation. (A) Western blotting showed that Fasudil injection decreased the expressions of ROCK, p-ERK1/2, YAP1, ETA, and ETB under mechanical pressure stimulated human vascular smooth muscle cells. The decreased expressions of ROCK, p-ERK1/2, YAP1, ETA, and ETB in Fasudil group were restored by ROCK-OE. (B) The quantitative analysis of relative protein levels uncovered that the expressions of ROCK, p-ERK1/2, YAP1, ETA, and ETB were increased in Fasudil group compared with those in Model group or Sham group. All P<0.05: Fasudil group vs. Model group or Sham group. The relative protein levels of ROCK, p-ERK1/2, YAP1, ETA, and ETB in Fasudil group were restored by ROCK-OE Lentivirus.