Research Paper Volume 14, Issue 16 pp 6642—6655

Figure 4. Cell viability, apoptosis, and caspase changes in 5637, T24, BIU 87, and SV-HUC-1 cells by treatment with non-toxic RH and TRAIL. Four bladder cell lines were treated with non-toxic RH (10 μg/mL) and different concentrations of TRAIL, TRAIL-sensitive cells (5637 cells, 5 ng/mL), and TRAIL-resistant cells (T24, BIU 87 and SV-HUC-1, 20 ng/mL) for 24 h. (A) Cell viabilities were measured by CCK8. (B) Cell apoptosis was detected via FCM. (C) The activation levels of Caspase-8 and Caspase-3 were detected via Western blot. Differences between groups were examined using one-way ANOVA. The results represent the means ± SD of three replicates, ^**P < 0.01, ^***P < 0.001.