Research Paper Volume 14, Issue 17 pp 6993—7002

Figure 2. Phps regulated the expression of p-ACC, p-AMPKβ, p-AKT, p- AMPKα, Nrf2, HO-1, GCLC, GCLM, NQO1 in APAP-induced acute liver injury of mice. The mice were received Phps (40, 60, 80 mg/kg) prior 1h APAP (500 mg/kg) injection and liver was collected for Western blot. (A), the expression of p-ACC, p-AMPKβ, p-AMPKα and p-AKT in liver of mice. (B–E), Relative expression levels of all proteins including p-ACC, ACC, p-AMPKβ, AMPKβ, p-AKT AKT p-AMPKα and AMPKα. (F), the expression of Nrf2, HO-1 in nuclear protein and the expression of Nrf2, GCLC, GCLM, NQO1 in cytoplasmic protein. (G–L), Relative expression levels of all proteins including Nrf2, HO-1 in nuclear protein and Nrf2, GCLC, GCLM, NQO1 in cytoplasmic protein. All data are presented as mean ± SD (three independent experiments). ^#p < 0.05 and ^##p < 0.01 vs. control group; *p < 0.05 and **p < 0.01 vs. APAP group.