Research Paper Volume 15, Issue 24 pp 15624—15639
A regulatory circuit of lncRNA NLGN1-AS1 and Wnt signalling controls clear cell renal cell carcinoma phenotypes through FZD4-modulated pathways
Figure 7. NLGN1-AS1 attenuates the proliferation abilities of ccRCC cells through FZD4/Wnt signaling pathway. (A) qRT-PCR was conducted to verify the relative expression of FZD4 in 769-P and Caki-1 cells transfected with sh-NLGN1-AS1 or oe-FZD4. (B) The expression of FZD4 was analyzed by western blotting with the indicated antibodies and samples from the 769-P and Caki-1 cells transfected with sh-NLGN1-AS1 or oe-FZD4. (C–E) CCK-8 assay, colony formation analysis, and EDU assay in 769-P and Caki-1 cells transfected with sh-NC+oe-NC, sh-NC+oe-FZD4 and sh-NLGN1-AS1+oe-FZD4. (F) In vivo evaluating in xenograft mouse models bearing tumors originating from 769-P cells stably transfected with sh-NC+oe-NC, sh-NC+oe-FZD4 and sh-NLGN1-AS1+oe-FZD4, n = 5/group; Tumor volume and weight of the xenograft were shown in the right. The data represent the mean ± SD of 3 replicates. *P < 0.05; **P < 0.01.