Figure 3. miR-450b-5p was high-expressed on renal IRI in rats. (A) The Gene Expression Omnibus (GEO) dataset obtained the potential differences of miRNAs by the gene expression matrices GSE75076, and GSE76549. (B) The heat map indicated the results of a two-way hierarchical clustering of the samples and found 58 differentially expressed miRNAs. (C) qRT-PCR assay showed the mRNA expression level of miR-450b-5p in SHAM, FLX, IRI and IRI+FLX groups. (D) FISH results showed the expression level of miR-450b-5p in SHAM group, and IRI group (magnification ×400). Data were presented as Mean ± SD, *significant difference vs. SHAM group (P < 0.05; **P < 0.01); #significant difference vs. IRI group (P < 0.05).