Research Paper Volume 14, Issue 18 pp 7527—7546

Ubiquitin-like protein FAT10 promotes renal fibrosis by stabilizing USP7 to prolong CHK1-mediated G2/M arrest in renal tubular epithelial cells

class="figure-viewer-img"

Figure 5. UUO-induced kidney fibrosis was suppressed in FAT10-deficient mice. (A and B) Representative HP and FAT10 staining in the kidneys from FAT10+/+ (A) and FAT10−/− (B) mice subjected to either UUO or sham operation; scale bar = 50 μm. (C and D) Representative Masson’s trichrome (C) and picrosirius red staining (D) of kidney sections from FAT10+/+ and FAT10−/− mice with or without UUO for 7 days. (E and F) Immunohistochemistry of protein expression of α-SMA (E) and Collagen IV (F) in obstructed kidneys from FAT10+/+ and FAT10−/− mice subjected to either UUO or sham operation. (G and H) Bar graph (right) shows quantification of fibrotic areas in histological sections; **P < 0.01 versus FAT10+/+ mice at the same time point; n = 6. (I and J) Bar graph shows quantification of areas of positive cells; **P < 0.01 versus FAT10+/+ mice at the same time point; n = 6.