Research Paper Volume 14, Issue 20 pp 8221—8242
AR12 increases BAG3 expression which is essential for Tau and APP degradation via LC3-associated phagocytosis and macroautophagy
Figure 16. Enhanced BAG3 expression requires autophagy and ER stress signaling. HCN2 cells were transfected with a scrambled siRNA control or with siRNA molecules to knock down the expression of Beclin1, ATG5, ULK1, eIF2α or PERK. After 24h, cells were treated for 6h with vehicle control or with AR12 (2 μM). Cells were fixed in place and immunostaining performed to detect the expression of BAG3 and ERK2 (n = 3 +/-SD) # p < 0.05 greater than vehicle control.