Research Paper Volume 15, Issue 1 pp 164—178

Figure 2. Doxorubicin increases expression and secretion of IGFBP3, and decreases cardiac cell survival in vitro and in vivo. (A) H9c2 cells were incubated with Dox for 24 h at the indicated doses and protein expression of IGFBP3 and that of the components of survival signaling pathway were measured using immunoblotting. (B) Left ventricles of control and Dox-administered rats were isolated and the levels of IGFBP3 and survival-related proteins were analyzed using western blotting. (C) IGFBP3 expression from the rat left ventricle tissue was detected using immunohistochemistry. (D) H9c2 cells were challenged with Dox for 24 h at indicated doses and the amount of secreted IGFBP3 in both cell medium and in the animal serum of Dox-treated rats was examined using western blotting. The quantitative plot of IGFBP3 from the sera of doxorubicin challenged rats is included. Scale bar indicates 200 μm.