Research Paper Volume 15, Issue 2 pp 542—552
Metformin increases bone marrow adipose tissue by promoting mesenchymal stromal cells apoptosis
Figure 4. Metformin increased bone marrow cell apoptosis in ob/ob mice. (A, B) TUNEL staining in control and metformin-treated mice tibia sections (scale bar = 200 mm). (C) Quantification of TUNEL-positive cells in the proximal tibia. (D) mRNA relative expression of apoptosis-related genes (Bax, Bcl-2, Bad) in the bone marrow. Data are presented as mean ± S.E.M. **P < 0.01, ***P < 0.001 vs. ob ctr.