Research Paper Volume 15, Issue 14 pp 6721—6735
Th1 promotes M1 polarization of intestinal macrophages to regulate colitis-related mucosal barrier damage
Figure 8. Suppressing STAT3 signaling antagonizes the effect of Th1 cells. (A, B) FCM (n=3). Stattic treatment antagonized Th1 cells, the proportion of M1 cells significantly decreased, and that of Th1+Stattic group was remarkably lower than that of Th1 group. *P<0.05 compared with Control group, #P<0.05 relative to Th1 group. (C) ELISA (n=3). The levels of M1 cell markers TNF-α, IL-6 and IL-1β significantly decreased in Th1+Stattic group, lower than those of Th1 group. *P<0.05 compared with Control group, #P<0.05 relative to Th1 group. (D, E) Protein detection (n=3). The expression levels of STAT3 and p-STAT3 in Th1+Stattic group significantly decreased. *P<0.05 compared with Control group, #P<0.05 relative to Th1 group.