Research Paper Volume 15, Issue 9 pp 3356—3380
Highly multiplexed immune profiling throughout adulthood reveals kinetics of lymphocyte infiltration in the aging mouse prostate
Figure 2. Unsupervised clustering of mouse urogenital immune cells identifies 29 phenotypically distinct clusters. (A) Heat map showing the phenotypes of the 29 clusters generated on immune cells detected from aging mouse prostates, bladders, and kidneys using CyTOF. Clusters were separated into T cells (CD3, CD4, CD8), B cells (B220, CD19), NK cells (CD335), myeloid cells (F4/80, CD11b, CD11c, and Ly6G), and unknown by expression of immune lineage markers. Shading represents mean marker expression transformed by arsinh(x). (B–E) Immune cell cluster expression of lineage markers for T cells (CD3) (B), B cells (B220) (C), NK cells (CD335) (D), and myeloid cells (CD11b and CD11c) (E). Data represents mean marker expression in each cluster transformed by arsinh(x) ± SD between clusters of the same broad immune cell type. Kruskal-Wallis, p < 0.001 (CD3), p < 0.05 (B220), p = 0.106 (CD335). Two-way ANOVA, p < 0.0001 (myeloid markers).