Research Paper Volume 15, Issue 10 pp 4182—4201

Figure 4. Sig1R mediates BEM-induced bladder cancer cell proliferation. (A) Sig1R expression levels were examined in Sig1R-KD and control bladder cancer cells (T24 and J82). (B) GSEA results showed that Sig1R expression was significantly associated with cell cycle-associated gene signatures, including “cell cycle,” “cell cycle G1–S phase transition,” “DNA replication,” and “cell cycle phase transition.” (C) Sig1R-KD and control bladder cancer cells (T24 and J82) were incubated with BEM hydrogel (0 and 20 mg/mL) for 2 weeks and were allowed to colonize. (D) The viability of Sig1R-KD and control bladder cancer cells (T24 and J82) incubated with BEM hydrogels (0 and 20 mg/mL) for 4 days was evaluated using the MTT assay. (E) the cell cycle distribution of Sig1R-KD and control bladder cancer cells (T24 and J82) treated with BEM hydrogels (0 and 20 mg/mL) for 48 h was evaluated by flow cytometry. (F) The percentage of cells in each phase is shown. ***p < 0.001.