Research Paper Volume 15, Issue 10 pp 4363—4373

PCSK6 mediates Th1 differentiation and promotes chronic colitis progression and mucosal barrier injury via STAT1

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Figure 4. Suppressing STAT1 inhibited Th1 cell differentiation. (A, B) FCM (n=3). Cirsilineol suppressed Th1 cell differentiation. The Th1 cells proportion remarkably decreased compared with those of PCSK6-OV, PCSK6-Si and Control groups. *P<0.05 vs. Control group, #P<0.05 vs. PCSK6-OV group. (C) ELISA (n=3). IL-2, IFN-γ, TNF-α, IL-6 and IL-1β contents of Cirsilineol group decreased relative to Control and PCSK6-OV groups, with no significant difference versus PCSK6-Si group. *P<0.05 vs. Control group, #P<0.05 vs. PCSK6-OV group. (D, E) Relative protein expression (n=3). IFN-γ, p-STAT1 and T-bet levels in Cirsilineol group remarkably decreased relative to PCSK6-OV group, with no significant difference versus PCSK6-Si group. *P<0.05 vs. Control group, #P<0.05 vs. PCSK6-OV group.