Research Paper Volume 15, Issue 10 pp 4411—4428

Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway

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Figure 3. The profiles of lncRNA H19, miR-194, and E2F3 in prostate cancer tissues and cell lines. (A, B) qRT-PCR detected lncRNA H19 expression in prostate cancer tissues and cell lines. (C, D) qRT-PCR determined miR-194 expression in prostate cancer tissues and cell lines. (E, F) qRT-PCR examined E2F3 mRNA expression in prostate cancer tissues and cell lines. (G, H) Western blot was performed for assaying E2F3 protein level. (I) Immunohistochemistry checked E2F3 expression in prostate cancer tissues. Scale bar = 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001 (vs. Normal or PrEC), n = 3.