Research Paper Volume 15, Issue 12 pp 5266—5278

Figure 1. Gene expression of selected H₂S production and removal enzymes. (A) Hepatic H₂S production capacity assay assessed in protein lysates from WT, G609G RC and G609G HFD mice. Data quantified by densitometry analysis of lead acetate assay results. A representative image of the lead sulfide precipitate that form as the output of the lead acetate assay is shown beneath the plot. Darker precipitates indicate higher hepatic H₂S producing enzymes Cse, Cbs, Mpst (B) and H₂S disposal enzymes Ethe1, Tst and Suox (C) as measured by RT-qPCR. Relative expression values were calculated using the 2^−ΔΔCt method. WT data shown in black, G609G RC in pink, G609G in Green. Statistical significance determined by one-sample t-test comparing the fold changes to a theoretical mean of one. Grubbs outlier test with alpha = 0.05 was performed, no outliers removed. Bars show mean values with error bars representing standard error of the mean. Abbreviations: Cse: Cystathionine-beta-lyase; Cbs: Cystathionine-Beta-synthase; Mpst: 3-Mercaptopyruvate Sulfurtransferase; Ethe1: Ethylmalonic encephalopathy 1 protein; Tst: Thiosulfate Sulfurtransferase; Suox: Sulfite Oxidase. ^*p < 0.05, ^**p < 0.01.