Research Paper Volume 15, Issue 18 pp 9438—9452

Figure 3. Fer-1 reversed APS-induced ferroptosis. (A) FerroOrange staining showed that Fer-1 could reduce the APS-induced increase in Fe²⁺ levels (Bar represents 20 μm, 20 × magnification). (B) Statistical analysis showed that Fer-1 reversed APS-induced Fe²⁺ (C) and MDA (D) accumulation in RT4 and T24 cells. Fer-1 reversed the APS-induced decrease in GSH levels (E) in RT4 and T24 cells. (F) CCK-8 analysis showed that the APS-induced decrease in the viability of RT4 and T24 cells could also be ameliorated by Fer-1. Flow cytometry revealed that Fer-1 treatment reduced the APS-induced increase in the mortality of RT4 (G, H) and (I, J) T24 cells. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. Con; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. APS.