Research Paper Volume 15, Issue 18 pp 9438—9452

Astragalus polysaccharide inhibits the development of urothelial carcinoma by activating AMPK signaling to induce BENC1-xCT complex formation


Figure 5. APS activates AMPK/BECN1 signaling in RT4 and T24 cells. (A) The Western blot results showed that APS significantly increased the phosphorylation levels of AMPK and BECN1 in RT4 and T24 cells. IF staining showed that APS induced the formation of the BECN1-xCT complex in RT4 (B) and T24 (C) cells (Bar represents 10 μm, 40 × magnification). **p < 0.01, ***p < 0.001 vs. Con.