Research Paper Volume 15, Issue 18 pp 9438—9452

Astragalus polysaccharide inhibits the development of urothelial carcinoma by activating AMPK signaling to induce BENC1-xCT complex formation


Figure 6. Knockdown of AMPK reverses APS-induced ferroptosis. (A) The Western blot results showed that the transfection of si-AMPK significantly inhibited AMPK expression even in APS-treated RT4 and T24 cells. (B) the JC-1 results showed that silencing AMPK significantly ameliorated APS-induced upregulation of MMP (Bar represents 20 μm, 20 × magnification). In RT4 and T24 cells, the APS-induced increase in Fe2+ (C) and MDA (D) levels could be alleviated by si-AMPK to some extent. (E) In RT4 and T24 cells, the reduction of GSH induced by APS could be reversed by knockdown of AMPK. *p < 0.05, ***p < 0.001 vs. Con; ##p < 0.01, ###p < 0.001 vs. APS.