Research Paper Volume 15, Issue 21 pp 11845—11859

Figure 6. CAP inhibited DOX-induced ferroptosis in H9C2 cells. (A) H9C2 cells were treated with 2.5 µM DMSO or DOX or 0.4 µM CAP+ 2.5 µM DOX for 6 hours, and then, qPCR was used for quantitative analysis of PTGS2 (right) and GPX4 (left) mRNA expression (n = 5). (B) Relative expressions of COX-2, SLC7All, FTHl, GPX4, TRF, SLC40Al in H9C2 (n = 3). (C) H9C2 cells were treated with 2.5 µM DMSO or DOX or 0.4 µM CAP+ 2.5 µM DOX for 8 or 12 hours, followed by extraction of proteins for Western blot analysis (n = 3). Data are shown as the mean ± SEM. Statistical significance was determined using two-tailed student’s t-test. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.